LC-MS Method Validation

26.11.2024 - 07.02.2025

Time of occurrence

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More information

Credits

2 ECTS (52 hours)

Form of study

Entirely web-based

Location

Veebis

Fee

free

Teachers

Ivo Leito
Koit Herodes
Karin Kipper
Riin Rebane
Irja Helm
Asko Laaniste
Hanno Evard

Study group

Chemistry

Program language

English

Purpose:

To offer comprehensive knowledge and skills in LC-MS Methods Validation.

Target group:

All who have preliminary knowledge about analytical chemistry, liquid chromatograpy and mass spectrometry.

Additional information

Outcome:

The participant who has successfully passed the course knows:
- the main performance parameters of analytical methods, what they show and which of them are particularly important in different situations;
- the main mathematical concepts and tools in method validation;
- the main approaches for evaluation of the performance parameters in the case of LC/MS analysis.
The participant who has successfully passed the course is able to:
- decide what data are needed for evaluating the different method performance parameters, understand the meaning of the available data and decide whether the available data are sufficient;
- select the approach and design the experiments for obtaining suitable data;
- quantify the relevant performance parameters using the available data and assess whether the obtained values are realistic;
- assess the fitness of the method for the intended purpose based on the values of the evaluated performance parameters.

Teachers:

Ivo Leito - https://analytical.chem.ut.ee/group/ivo-leito/
Koit Herodes - https://www.etis.ee/CV/Koit_Herodes/est?lang=ENG&tabId=CV_ENG
Karin Kipper - https://www.etis.ee/CV/Karin_Kipper/est?tabId=CV_ENG
Riin Rebane - https://www.etis.ee/CV/Riin_Rebane/est?tabId=CV_ENG
Irja Helm - https://www.etis.ee/CV/Irja_Helm/est?tabId=CV_ENG
Asko Laaniste - https://www.ut.ee/et/asko-laaniste https://www.linkedin.com/in/asko-laaniste-8a70767a/
Hanno Evard - https://www.ut.ee/et/hanno-evard

Content:

Topic	Credit	Lectors
1. Practice-oriented on-line course on validation of analytical methods, specifically using LC-MS as technique. The course covered the following topics: - The concept, workflow and scope of validation; - Selectivity and identity confirmation, both via LC and via MS; - Linearity of signal, linear range, sensitivity and their relation to calibration; - Precision, trueness, accuracy, analyte stability and their interrelations; - Limit of detection and limit of quantitation; - Ruggedness and robustness.	52	Ivo Leito, Koit Herodes, Karin Kipper, Riin Rebane, Irja Helm, Asko Laaniste, Hanno Evard

Price info:

Free MOOC
The students who have successfully passed the course will be issued a certificate of completion by the University of Tartu.
A digital certificate of completion is free of charge. Please read the technical requirements for downloading and opening the digitally verified document: https://ut.ee/en/digital-continuing-education-certificates

A certificate of completion on paper can be requested for a fee of 61 euros (incl. all taxes). The basis of payment for the certificate on paper is the invoice issued by the university. The certificate will be sent out after the receipt of payment.

If you would like to get a paper certificate, please provide us with this information before the end of the course by e-mail: Esta.pilt@ut.ee.

Location info:

Web-based course in Moodle.

Learning environment:

Web-based course in Moodle.

Schedule and further information:

According to the action plan in Moodle. There are no live lectures.
For every week a set of tasks will be given.
At the end of each week there will be a graded test (in the Moodle environment) and at the end of the course there is final test.

Requirements to complete:

Passing all graded tests with at least 50% of the maximum score.

Outcome method:

differentiated (A, B, C, D, E, F, not present)

Grading method:

tests

Grading criteria:

Passing all graded tests with at least 50% of the maximum score.

Document to be issued:

Certificate of completion

Registration information:

Please enter the correct full name and date of birth during registration.They go on the final document.